Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Exogenous insulin‐like growth factor 1 attenuates cisplatin‐induced muscle atrophy in mice

doi: 10.1002/jcsm.12760

Figure Lengend Snippet: Effect of insulin‐like growth factor‐1 (IGF‐1) on cisplatin‐induced changes in IGF‐1/Akt/mammalian target of rapamycin and Smad signalling in C2C12 myotubes. Representative photographs of western blot bands for phospho‐Akt, total‐Akt, phospho‐p70S6 kinase, total‐p70S6 kinase and GAPDH (A) . The data are summarized in (B) and (C) . Representative photographs of western blot bands for phospho‐Foxo3a, total‐Foxo3a, phospho‐pSmad2/pSmad3, phospho‐pSmad2, total‐Smad2 and GAPDH (D) . Data are summarized in (E) and (F) . Each column represents the mean ± SD from three independent experiments. ** P < 0.01 and *** P < 0.001 vs. vehicle control (control). # P < 0.05 and ### P < 0.001 vs. Cis15 (cisplatin 15 μM).

Article Snippet: The following primary rabbit antibodies were used: anti‐IGF‐1 (1:1000 dilution; PeproTech), anti‐IGF‐1R beta chain (1:1000 dilution; Proteintech Group, Inc., IL, USA), anti‐MuRF1 (1:1000 dilution; ECM Biosciences, Versailles, KY, USA), anti‐atrogin‐1 (1:1000 dilution; ECM Biosciences), anti‐Foxo3a (1:1000 dilution; Cell Signaling Technology, Danvers, MA, USA), anti‐Akt (1:1000; Cell Signaling Technology), anti‐phospho‐Akt (1:1000; Cell Signaling Technology), anti‐phospho‐Foxo3a (1:1000; Cell Signaling Technology), anti‐Smad2 (1:1000; Cell Signaling Technology) anti‐phospho‐Smad2/Smad3 (1:1000; Cell Signaling Technology), and anti‐phospho‐Smad2 (1:1000; Cell Signaling Technology).

Techniques: Western Blot

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Exogenous insulin‐like growth factor 1 attenuates cisplatin‐induced muscle atrophy in mice

doi: 10.1002/jcsm.12760

Figure Lengend Snippet: Effect of mecasermin on cisplatin‐induced changes in insulin‐like growth factor 1 (IGF‐1)/Akt/mammalian target of rapamycin and Smad signalling in mouse quadriceps muscle. Representative photographs of western blot bands for phospho‐Akt, total‐Akt, phospho‐p70S6 kinase, total‐p70S6 kinase and GAPDH (A) . Data are summarized in (B) and (C) . Representative photographs of western blot bands for phospho‐Foxo3a, total‐Foxo3a, phospho‐pSmad2, total‐Smad2 and GAPDH (D) . Data are summarized in (E) and (F) . Each column represents the mean ± SD from four or five mice per group. ** P < 0.01 and *** P < 0.001 vs. vehicle control (Saline + Saline). # P < 0.05 and ### P < 0.001 vs. Saline + Cis3 (cisplatin 3 mg/kg).

Article Snippet: The following primary rabbit antibodies were used: anti‐IGF‐1 (1:1000 dilution; PeproTech), anti‐IGF‐1R beta chain (1:1000 dilution; Proteintech Group, Inc., IL, USA), anti‐MuRF1 (1:1000 dilution; ECM Biosciences, Versailles, KY, USA), anti‐atrogin‐1 (1:1000 dilution; ECM Biosciences), anti‐Foxo3a (1:1000 dilution; Cell Signaling Technology, Danvers, MA, USA), anti‐Akt (1:1000; Cell Signaling Technology), anti‐phospho‐Akt (1:1000; Cell Signaling Technology), anti‐phospho‐Foxo3a (1:1000; Cell Signaling Technology), anti‐Smad2 (1:1000; Cell Signaling Technology) anti‐phospho‐Smad2/Smad3 (1:1000; Cell Signaling Technology), and anti‐phospho‐Smad2 (1:1000; Cell Signaling Technology).

Techniques: Western Blot

Journal: Contrast Media & Molecular Imaging

Article Title: Radiosynthesis and Preclinical Evaluation of 11 C-VA426, a Cyclooxygenase-2 Selective Ligand

doi: 10.1155/2019/5823261

Figure Lengend Snippet: Semipreparative HPLC purification of 11 C-VA426 radiolabelling.

Article Snippet: Acetonitrile extracts were centrifuged and supernatant filtered with a MillexGX® syringe system for HPLC (Gilson Italia Srl, 321 pump, UV/VIS-151) injection.

Techniques: Purification

Journal: Contrast Media & Molecular Imaging

Article Title: Radiosynthesis and Preclinical Evaluation of 11 C-VA426, a Cyclooxygenase-2 Selective Ligand

doi: 10.1155/2019/5823261

Figure Lengend Snippet: Analytical HPLC quality control. (a) UV chromatogram of the reference standard VA426; (b) UV chromatogram of 11 C-VA426; (c) radiochromatogram of 11 C-VA426 showing a radiochemical purity ≥95%.

Article Snippet: Acetonitrile extracts were centrifuged and supernatant filtered with a MillexGX® syringe system for HPLC (Gilson Italia Srl, 321 pump, UV/VIS-151) injection.

Techniques: Control

Journal: Contrast Media & Molecular Imaging

Article Title: Radiosynthesis and Preclinical Evaluation of 11 C-VA426, a Cyclooxygenase-2 Selective Ligand

doi: 10.1155/2019/5823261

Figure Lengend Snippet: Stability study of 11 C-VA426 by HPLC analysis, in the plasma of healthy mice ( n = 3), 10 min p.i.

Article Snippet: Acetonitrile extracts were centrifuged and supernatant filtered with a MillexGX® syringe system for HPLC (Gilson Italia Srl, 321 pump, UV/VIS-151) injection.

Techniques: Clinical Proteomics

Journal: Contrast Media & Molecular Imaging

Article Title: Radiosynthesis and Preclinical Evaluation of 11 C-VA426, a Cyclooxygenase-2 Selective Ligand

doi: 10.1155/2019/5823261

Figure Lengend Snippet: Stability study of 11 C-VA426 by HPLC analysis, in the liver of healthy mice ( n = 3), 10 min p.i.

Article Snippet: Acetonitrile extracts were centrifuged and supernatant filtered with a MillexGX® syringe system for HPLC (Gilson Italia Srl, 321 pump, UV/VIS-151) injection.

Techniques: